Methotrexate Analysis

Specimen Tube Type Transport

Non Gel Red Top or Gel Green Top

Container Whole Blood Required

7 ml whole blood in a Non Gel Red Top or Green Gel Top

Critical Value

Following completion of high dose Methotrexate infusion, Methotrexate levels are considered toxic if:
>5.00 umol/L at 24 hours

Summary Test Name

Methotrexate Analysis

Specimen Preparation Outreach

Spin right away, separate plasma into plastic 12 x 75 pour-off tube. Cap with plastic stopper. Refrigerate until courier pickup.

Specimen Stability

2 weeks refrigerated, if spun and plasma removed from gel; 6 hours on the gel

Specimen Transport

Refrigerate

Specimen Preparation In House

Spin/separate and refrigerate until analysis

Stat Availability

24 hours a day, seven days a week

Test Turnaround Time Routines And Stats

Routine 4 hours, Stat 1 hour

Reference Value

Variable